Articles

VPCIR™ technology is based on years of research within the field of DNA modifying enzymes and their use in both detection and manipulation of cell activity.

You can read more on the different VPCIR™ technology solutions in the following documentation:


VPCIR™ technology – general

Keller JG, Mizielinski K, Petersen KV, Stougaard M, Knudsen BR, Tesauro C. Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples. J Vis Exp. 2022. doi: 10.3791/64484.

Keller, JG, Petersen KV, Knudsen BR, Tesauro C. Simple and Fast DNA-Based Tool to Investigate Topoisomerase 1 Activity, a Biomarker for Drug Susceptibility in Colorectal Cancer. Recent Understanding of Colorectal Cancer Treatment, IntechOpen. 2022. doi: 10.5772/intechopen.105758.

Petersen KV, Tesauro C, Hede MS, Pages C, Marcussen LB, Keller JG, Bugge M, Holm K, Bjergbæk L, Stougaard M, Wejse C, Knudsen BR. Rolling Circle Enhanced Detection of Specific Restriction Endonuclease Activities in Crude Cell Extracts. Sensors (Basel). 2022. doi: 10.3390/s22207763.

Jakobsen AK, Keller JG, Gonzalez M, Martin-Encinas E, Palacios F, Alonso C, Knudsen BR, Stougaard M. A Dual-Sensor-Based Screening System for In Vitro Selection of TDP1 Inhibitors. Sensors. 2021. doi: 10.3390/s21144832.

Petersen KV, Selas A, Hymøller KM, Mizielinski K, Thorsager M, Stougaard M, Alonso C, Palacios F, Pérez-Pertejo Y, Reguera RM, Balaña-Fouce R, Knudsen BR, Tesauro C. Simple and Fast DNA Based Sensor System for Screening of Small-Molecule Compounds Targeting Eukaryotic Topoisomerase 1. Pharmaceutics. 2021. doi: 10.3390/pharmaceutics13081255.

Keller JG, Stougaard M, Knudsen BR. Enzymatic activity in single cells. Methods Enzymol. 2019. doi: 10.1016/bs.mie.2019.07.003.

Hede MS, Fjelstrup S, Lötsch F, Zoleko RM, Klicpera A, Groger M, Mischlinger J, Endame L, Veletzky L, Neher R, Simonsen AKW, Petersen E, Mombo-Ngoma G, Stougaard M, Ho YP, Labouriau R, Ramharter M, Knudsen BR. Detection of the Malaria causing Plasmodium Parasite in Saliva from Infected Patients using Topoisomerase I Activity as a Biomarker. Sci Rep. 2018. doi: 10.1038/s41598-018-22378-7.

Keller JG, Tesauro C, Coletta A, Graversen AD, Ho YP, Kristensen P, Stougaard M, Knudsen BR. On-slide detection of enzymatic activities in selected single cells. Nanoscale. 2017. doi: 10.1039/c7nr05125e.

Givskov A, Kristoffersen EL, Vandsø K, Ho YP, Stougaard M, Knudsen BR. Optimized Detection of Plasmodium falciparum Topoisomerase I Enzyme Activity in a Complex Biological Sample by the Use of Molecular Beacons. Sensors (Basel). 2016. doi: 10.3390/s16111916.

Juul S, Nielsen CJ, Labouriau R, Roy A, Tesauro C, Jensen PW, Harmsen C, Kristoffersen EL, Chiu YL, Frøhlich R, Fiorani P, Cox-Singh J, Tordrup D, Koch J, Bienvenu AL, Desideri A, Picot S, Petersen E, Leong KW, Ho YP, Stougaard M, Knudsen BR. Droplet microfluidics platform for highly sensitive and quantitative detection of malaria-causing Plasmodium parasites based on enzyme activity measurement. ACS Nano. 2012. doi: 10.1021/nn3038594.

Tesauro C, Juul S, Arnò B, Nielsen CJ, Fiorani P, Frøhlich RF, Andersen FF, Desideri A, Stougaard M, Petersen E, Knudsen BR. Specific detection of topoisomerase I from the malaria causing P. falciparum parasite using isothermal rolling circle amplification. Annu Int Conf IEEE Eng Med Biol Soc. 2012. doi: 10.1109/EMBC.2012.6346451

Juul S, Ho YP, Koch J, Andersen FF, Stougaard M, Leong KW, Knudsen BR. Detection of single enzymatic events in rare or single cells using microfluidics. ACS Nano. 2011. doi: 10.1021/nn203012q.

Andersen FF, Stougaard M, Jørgensen HL, Bendsen S, Juul S, Hald K, Andersen AH, Koch J, Knudsen BR. Multiplexed detection of site specific recombinase and DNA topoisomerase activities at the single molecule level. ACS Nano. 2009. doi: 10.1021/nn9012912.

 

VPCIR™ EAD

Franch O , Han X , Marcussen LB , Givskov A , Andersen MB , Godbole AA , Harmsen C , Nørskov-Lauritsen N , Thomsen J , Pedersen FS , Wang Y , Shi D , Wejse C , Pødenphant L , Nagaraja V , Bertl J , Stougaard M , Ho YP , Hede MS , Labouriau R , Knudsen BR . A new DNA sensor system for specific and quantitative detection of mycobacteria. Nanoscale. 2019. doi: 10.1039/c8nr07850e.

Detection of endonuclease activity. PCT/EP2019/058478

 

VPCIR™ REEAD TOP1 Activity (including REEAD C|L)

Keller JG, Mizielinski K, Petersen KV, Stougaard M, Knudsen BR, Tesauro C. Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples. J Vis Exp. 2022. doi: 10.3791/64484.

Keller, JG, Petersen KV, Knudsen BR, Tesauro C. Simple and Fast DNA-Based Tool to Investigate Topoisomerase 1 Activity, a Biomarker for Drug Susceptibility in Colorectal Cancer. Recent Understanding of Colorectal Cancer Treatment, IntechOpen. 2022. doi: 10.5772/intechopen.105758.

Jakobsen AK, Keller JG, Gonzalez M, Martin-Encinas E, Palacios F, Alonso C, Knudsen BR, Stougaard M. A Dual-Sensor-Based Screening System for In Vitro Selection of TDP1 Inhibitors. Sensors. 2021. doi: 10.3390/s21144832.

Petersen KV, Selas A, Hymøller KM, Mizielinski K, Thorsager M, Stougaard M, Alonso C, Palacios F, Pérez-Pertejo Y, Reguera RM, Balaña-Fouce R, Knudsen BR, Tesauro C. Simple and Fast DNA Based Sensor System for Screening of Small-Molecule Compounds Targeting Eukaryotic Topoisomerase 1. Pharmaceutics. 2021. doi: 10.3390/pharmaceutics13081255.

Tesauro C, Keller JG, Gromova I, Gromov P, Frohlich R, Erlandsen JU, Andersen AH, Stougaard M, Knudsen BR. Different Camptothecin Sensitivities in Subpopulations of Colon Cancer Cells Correlate with Expression of Different Phospho-Isoforms of Topoisomerase I with Different Activities. Cancers (Basel). 2020. doi: 10.3390/cancers12051240.

EP2021/21164120.4