It is often hypothesized that the cytotoxic effect of anti-cancer chemotherapeutics from the camptothecin family, such as irinotecan and topotecan, corresponds to the TOP1 activity level in cancer cells, tissues or biopsies.
The REEAD TOP1 activity kit was used to measure the TOP1 activity level in a panel of cell lines derived from different breast cancer subtypes including Luminal, HER2 positive or Triple Negative Breast Cancer (TNBC). The chemosensitivity of the cells was correlated to TOP1 activity level, TOP1 expression, TOP1 gene copy number, and cellular growth rate.
The study demonstrated that the chemoresponse of the breast cancer derived cells lines correlated directly to the cell division rate and for one breast cancer subtype, namely TNBC, which are currently difficult to treat, the chemoresponse correlated also to the TOP1 activity level.
BMC cancer, 2019. Doi: 10.1186/s12885-019-6371-0
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