Monkeypox Virus Topoisomerase 1 Enzyme

Monkeypox Virus Topoisomerase 1 Enzyme


Regular price€550,00
Shipping calculated at checkout.

  • In stock, ready to ship
  • Inventory on the way

Want a custom product?

Please contact us for a quote

High purity, catalytically active monkeypox virus topoisomerase 1 enzyme overexpressed in yeast and purified to single band homogeneity.


Uses Research in cancer, research in chronic diseases, research in infectious diseases – for research use only. Not for use in diagnostic procedures
Purity 80%
Sizes 1.5 µg ≥ 1,500 units, 3.5 µg ≥ 3,500 units, 10 µg ≥ 10,000 units
Variants Single-pack, 2-pack, 3-pack
Shipping Not IATA restricted
Storage Must be stored at -18°C to -20°C upon arrival

We ship with DHL Medical Express.

All orders are in sent stylefoam boxes with frozen gel packages.

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Monkeypox Virus topoisomerase 1 enzyme is supplied in Dilution Buffer1 at a minimum concentration of 30ng/µl, corresponding to at least 30 U/µl. 1 U of mvpTOP1 relaxes 0.5 µg of supercoiled pUC19 when incubated in 1xReaction Buffer2 and 150 mM NaCl for 30 minutes at 37°C – see Documentation.

The enzyme preparation is highly stable and recommended for activity assays, including cleavage-ligation experiments, equilibrium, nicking, and relaxation assays.

The enzyme is supplied together with:
• Dilution Buffer: 5 mM Tris-HCl pH 7.5, 400 mM NaCl, 1mM DTT, 50% glycerol
• Reaction Buffer 10x: 100 mM Tris-HCl pH 7.5; 50 mM MgCl2, 50 mM CaCl2; 50 µg/ml BSA
• Nucleases-free water
• 1M NaCl

Recombinant 6HIS-tagged monkeypox virus topoisomerase 1 is prepared by overexpressing the enzyme in Saccharomyces cerevisiae followed by purification by affinity chromatography adapted from Knudsen et al, J. Chrom. 1996, DOI: 10.1016/0378-4347(96)00152-1

The enzyme is shipped packed with ice gel packs and must be stored at -18°C to -20°C upon arrival. The temperature must be controlled to avoid that the enzyme preparation solidifies. This would destroy the enzyme integrity and cause a drop of the monkeypox virus topoisomerase 1 activity. The enzyme has a lifetime of 12 months when stored under optimal conditions

Certificate of Analysis for Monkeypox Virus topoisomerase 1

Certificate of Analysis for Monkeypox Virus topoisomerase 1

Safety Data Sheet for Monkeypox Virus topoisomerase 1 enzyme products

Safety Data Sheet for Monkeypox Virus topoisomerase 1 enzyme products

Coming soon

1. Estep, R.D.; Messaoudi, I.; O’Connor, M.A.; Li, H.; Sprague, J.; Barron, A.; Engelmann, F.; Yen, B.; Powers, M.F.; Jones, J.M.; et al. Deletion of the Monkeypox Virus Inhibitor of Complement Enzymes Locus Impacts the Adaptive Immune Response to Monkeypox Virus in a Nonhuman Primate Model of Infection. J. Virol. 2011, 85, 9527–9542, doi:10.1128/jvi.00199-11.

2. Shuman, S. Vaccinia virus DNA topoisomerase: A model eukaryotic type IB enzyme. Biochim. Biophys. Acta – Gene Struct. Expr. 1998, 1400, 321–337, doi:10.1016/S0167-4781(98)00144-4.



Recently viewed