VPCIRTM stands for Viability Polymerase Circle Reaction. It is based on the principle of detection of enzymatic processes allowing quantification of the activity of specific DNA-modifying enzymes. This is done by providing a substrate that the DNA modifying enzyme recognize and either directly or via additional enzymes turn into a circle which subsequently are amplified and visualized. The technology may be used for various purposes such as drug screening, cancer research, or for detection of pathogens.
Enzymes are biomolecules catalyzing specific biochemical reactions. Thus, the biological relevance depends on the ability to catalyze these reactions, id est, the activity of the enzymes. Unlike PCR and ELISA methods, each target enzyme can convert many DNA substrate molecules into detectable DNA product molecules up to 500-1,000 conversions per target enzyme. Thus, the sensitivity is greatly increased both through the inherent target enzyme catalyzed conversion of substrates to products and through a subsequent isothermal amplification reaction of the generated products.
The patent pending VPCIRTM technology is based on the knowledge from more than 10 years of research in specific sensor-based assays and more than 20 years of research in DNA modifying enzymes within the VPCIR founder team. The technology is robust and capable of accurate measurements using various inputs such as recombinant enzymes for drug screening, crude cell extract as well as tissue extract for biological and anti-cancer research, blood or saliva for pathogen detection, and food-borne bacteria detected within complex food matrices as milk and meat. The technology behind is sensitive enough to detect single cells and can easily be adapted to detect multiple pathogens (multiplexed).
Uses and Users
VPCIR ™ technology is utilized in several core technology platforms:
VPCIR ™ REEAD
VPCIR ™ EAD
VPCIR ™ IAD
VPCIR ™ Enzymes
Each with different uses and users.
See detail on each technology in subsequent tabs in the menu on top.
We use cookies to optimize our website and our service.
Functional
Always active
The technical storage or access is strictly necessary for the legitimate purpose of enabling the use of a specific service explicitly requested by the subscriber or user, or for the sole purpose of carrying out the transmission of a communication over an electronic communications network.
Preferences
The technical storage or access is necessary for the legitimate purpose of storing preferences that are not requested by the subscriber or user.
Statistics
The technical storage or access that is used exclusively for statistical purposes.The technical storage or access that is used exclusively for anonymous statistical purposes. Without a subpoena, voluntary compliance on the part of your Internet Service Provider, or additional records from a third party, information stored or retrieved for this purpose alone cannot usually be used to identify you.
Marketing
The technical storage or access is required to create user profiles to send advertising, or to track the user on a website or across several websites for similar marketing purposes.
We use cookies to optimize our website and our service.
Functional
Always active
The technical storage or access is strictly necessary for the legitimate purpose of enabling the use of a specific service explicitly requested by the subscriber or user, or for the sole purpose of carrying out the transmission of a communication over an electronic communications network.
Preferences
The technical storage or access is necessary for the legitimate purpose of storing preferences that are not requested by the subscriber or user.
Statistics
The technical storage or access that is used exclusively for statistical purposes.The technical storage or access that is used exclusively for anonymous statistical purposes. Without a subpoena, voluntary compliance on the part of your Internet Service Provider, or additional records from a third party, information stored or retrieved for this purpose alone cannot usually be used to identify you.
Marketing
The technical storage or access is required to create user profiles to send advertising, or to track the user on a website or across several websites for similar marketing purposes.